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This book describes a new family of bio-polymer§gels made from cytoskeletal proteins - actin, microtubule, and§tropomyosin. The importance of the gel§state with multi-scale hierarchical structure is emphasized to utilize emergent§functions in living organisms. Detailed protocol of gel preparation, specified§method of structure investigation, and dynamic studies of self-organization,§self-healing, synchronized oscillating, and autonomous motility functions are§introduced together with biomimetic§functions of synthetic hydrogels.§§