Characterisation of immune proteins from Clarias sp. and Pangasius sp

Aquaculture was introduced in the Sub-Sahara as an innovation to improve economies and nutrition of local communities. After years of local and international investment, aquaculture is still struggling with basic issues such as limited personnel, poor training in aquaculture technologies and practice, lack of appropriate aquaculture species and diseases. This book sets out to describe the methodology of isolating and characterising immune proteins (lectins and immunoglobulin mu (IgM)) from the African cat fish, Clarias gariepinus and Pangasius hypophthalmus which is a basis for developing tools for monitoring fish health. More than one affinity chromatography isolation steps are required to isolate Serum Amyloid Protein, C-reactive protein, mannose and galactose binding proteins. The purified fractions migrate as more than one band in Tris-tricine SDS-PAGE in the presence of beta-mercaptoethanol. On the other hand IgM isolated from P. hypophthalmus seem to use a single recognition site to bind to fucose and GlcNAc. The implication of these findings can not be ignored when considering using these sugar binding lectins and IgM as tools for monitoring fish health.